APA
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Izadifar, Z., Cotton, J., Chen, S., Horváth, V., Stejskalová, A., Gulati, A., … Ingber, D. E. (2024). Mucus production, host-microbiome interactions, hormone sensitivity, and innate immune responses modeled in human cervix chips. Nature Communications.
Chicago/Turabian
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Izadifar, Z., Justin Cotton, Siyu Chen, Viktor Horváth, A. Stejskalová, Aakanksha Gulati, Nina LoGrande, et al. “Mucus Production, Host-Microbiome Interactions, Hormone Sensitivity, and Innate Immune Responses Modeled in Human Cervix Chips.” Nature Communications (2024).
MLA
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Izadifar, Z., et al. “Mucus Production, Host-Microbiome Interactions, Hormone Sensitivity, and Innate Immune Responses Modeled in Human Cervix Chips.” Nature Communications, 2024.
BibTeX Click to copy
@article{z2024a,
title = {Mucus production, host-microbiome interactions, hormone sensitivity, and innate immune responses modeled in human cervix chips},
year = {2024},
journal = {Nature Communications},
author = {Izadifar, Z. and Cotton, Justin and Chen, Siyu and Horváth, Viktor and Stejskalová, A. and Gulati, Aakanksha and LoGrande, Nina and Budnik, Bogdan and Shahriar, S. and Doherty, Erin R. and Xie, Yixuan and To, Tania and Gilpin, S. and Sesay, A. and Goyal, Girija and Lebrilla, C. and Ingber, Donald E.}
}
Modulation of the cervix by steroid hormones and commensal microbiome play a central role in the health of the female reproductive tract. Here we describe organ-on-a-chip (Organ Chip) models that recreate the human cervical epithelial-stromal interface with a functional epithelial barrier and production of mucus with biochemical and hormone-responsive properties similar to living cervix. When Cervix Chips are populated with optimal healthy versus dysbiotic microbial communities (dominated by Lactobacillus crispatus and Gardnerella vaginalis, respectively), significant differences in tissue innate immune responses, barrier function, cell viability, proteome, and mucus composition are observed that are similar to those seen in vivo. Thus, human Cervix Organ Chips represent physiologically relevant in vitro models to study cervix physiology and host-microbiome interactions, and hence may be used as a preclinical testbed for development of therapeutic interventions to enhance women’s health. Human cervical mucosa and its interactions with the microbiome play a central role in female reproductive tract health and disease. Here, the authors develop physiological models of the human cervix using Organ-on-a-Chip technology that produce mucus, and respond to hormonal, environmental, and microbial cues similar to the living cervix.